Liquid chromatography

Our laboratory is equipped with several liquid chromatography (LC) systems. Each system is coupled with a unique detection system, which makes it possible to analyse a broad range of organic chemicals in samples of various origins. In addition to identification and quantitation of chemicals, development of new (LC) methodology is an integral part of the services provided by our laboratory.

HPLC-UV/Flu

HPLC-UV/Flu

Our high performance liquid chromatograph system is equipped with both a general purpose UV detector and highly sensitive and selective fluorescence detector (HPLC-UV/Flu). The system is typically used for the analysis of a wide variety of water soluble compounds within natural samples (e.g. water, soil and plants) or used in degradation or sorption studies. Typical applications include PAH analysis for bioremediation and sorption experiments, plant pigments and toxins, (degradation products of) herbicides and fullerenes, and phenolic acids within soil.

Technical Specifications

  • Chromatography: HPLC Prominence, equipped with a binary LC-20AD pumping system, SIL-20 autosampler and CTO-20 column oven (Shimadzu, Kyoto, Japan).
  • Detection: SPD-M20A photodiode array detector (PDA) and RF-10 XL fluorescence detector (Shimadzu, Kyoto, Japan).

LC-MS/MS

LC-MS/MS

Our triple-quadrupole like LC-MS/MS system is used for quantitative monitoring of a wide variety of chemicals in the environment. The system is equipped with a highly selective and sensitive tandem mass spectrometer (MS/MS), which can be utilized with different ionization techniques (ESI, APCI and APPI) to analyse a wide range of chemicals. Typical analyses within IBED include monitoring of perfluoroalkyl acids (PFAAs) within water, food and plant material, degradation studies of fullerenes, aryl organophosphorus flame retardants (OPFRs) and several herbicides, monitoring of azaarenes and azaarones within sediment, and the analysis of amino acids within soil.

Technical Specifications

  • Chromatography: HPLC Prominence XR, equipped with a binary LC-20AD XR gradient pumping system, SIL-20AC autosampler, CTO-20AC column oven and CBM-20A controller (Shimadzu, Kyoto, Japan).
  • Detection: 4000 QTRAP MS/MS (Applied Biosystems, Toronto, Canada).

UPLC-QTOF

UPLC-QTOF

A recent addition to our chromatography facilities is our ultra-performance liquid chromatography quadrupole time of flight (UPLC-QTOF) system. The combination of high chromatographic resolution, mass resolving power and mass accuracy, MS/MS and isotopic pattern fingerprinting, allows this instrument to identify and characterize chemicals even within complex sample material. The system can be equipped with several ion sources (ESI, APCI, APPI and IonBooster). Furthermore, Direction Injection Probe (DIP) can be used for direct analysis of both solid and liquid samples. Recent work includes the characterization of (degraded) perfluorinated polymers and screening for degradation products of aryl organophosphorus flame retardants.

Technical Specifications

  • Chromatography: Nexera UHPLC system (Shimadzu, Kyoto, Japan), equipped with a binary LC-30AD gradient pumping system, a LC-20AD dopant delivery pump, SIL-30AC autosampler and CTO-20AC column oven.
  • Detection: SPD20-A UV detector (Shimadzu, Kyoto, Japan) and maXis 4G Q-TOF MS (Bruker Daltonics, Bremen, Germany).

Published by  IBED

3 February 2014