A research article written by Prof. Gerard Muyzer has been listed as the highest cited paper of the Microbiology research field. The article describes a molecular approach to analyse the genetic diversity of complex microbial populations and has been cited 7558 times since it was published in 1993.
The research article written by Gerard Muyzer, Ellen de Waal and Andre Uitterlinden describes a technique that is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). Over the last 25 years DGGE has been frequently used to analyse the genetic diversity of complex microbial populations. This has resulted in a large number of citations of the research article that was originally published in Applied and Environmental Microbiology.
The research paper by Muyzer et al. was listed as the top cited paper in Microbiology by the authors of a recent research letter that lists the highest cited papers in Microbiology (Moral-Munoz et al. 2018). The research letter, published in the scientific journal FEMS Microbiology letters, applied bibliometric methods to identify the highest cited papers that contributed to the development of Microbiology. The Netherland was mentioned as one of the countries that made a relatively high scientific effort in the field of Microbiology.
The research article on the DGGE method was published back in 1993 when Gerard Muyzer and colleagues were working at Leiden University. Gerard Muyzer, who is honoured that the paper made such a big contribution to the field, is currently professor of Microbial Systems Ecology at the UvA’s Institute for Biodiversity and Ecosystem Dynamics.
Gerard Muyzer, Ellen C. de Waal and Andre G. Uitterlinden (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology Vol. 59 No. 3.
Jose A. Moral-Munoz, David Lucena-Antón, Verónica Perez-Cabezas, Inés Carmona-Barrientos, Gloria González-Medina and Carmen Ruiz-Molinero (2018). Highly cited papers in Microbiology: identification and conceptual analysis. FEMS Microbiology Letters, 365.