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Greenhouses, climate chambers and insect culture facilities

IBED has multiple greenhouses, climate-controlled rooms and cabinets to perform experiments in controlled conditions.

greenhouse
Inside the climate chamber. Photo: IBED

Climate chambers

The 25 Climate chambers at IBED are divided in five blocks across three floors in the B-building at Science Park to separate organisms from different trophic levels; plants, herbivores and predators. Each block has a sink for cleaning materials and is adjacent to a small ancillary lab for processing materials. 

Each climate chamber can be adjusted on-line in an accurate manner from a wide range of temperature, humidity and light conditions. Furthermore, 6 of the 25 climate chambers are equipped with so called lamps “Zonnehemel” that allows to reach light conditions (intensity and quality) comparable to solar light. These climate chambers are also used for small-scale bioassays and experiments under controlled conditions. 

Some climate rooms have the necessary security measurements that allow working with genetically modified plants and arthropods.

Quarantine tent. Photo: IBED

Insect culture facilities

Moth lab

We have a dedicated quarantine lab for the culturing of many different species of nocturnal moths that are invasive pests. The moths are used in pheromone related behavioural experiments, genetic analysis, and in experiments related to immune response and genetic interactions with species specific parasites.

The lab is fully adapted to the strict rules that are needed to work with quarantine insects and CRISPR-cas genetically modified lines.

Size comparison of Aculops lycopersici, which is the smallest terrestrial arthropod, sitting on the compound eye of a moth Heliothis subflexa. Photo: IBED

Thrips and mite labs

Each climate room set has an adjacent lab featured with necessary equipment like binoculars, microscopes and leaf scanner to setting up plants and arthropod cultures, performing behavioural observations, harvesting samples for –omics studies (genomic; transcriptomic and metabolomics), and acquisition of high-definition pictures to analyse herbivory damage and performing ultra-macro photography.

We also have a Potter Spray Tower®, which is internationally recognized as the most precise method of chemical spraying in the laboratory.

Climate cabinets. Photo: IBED

Climate cabinets

In total we have 14 MLR-352H-PE versatile Environmental Test Chambers and 3 MIR-254-PE Cooled incubators (PHCBI, formally known as Sanyo/Panasonic). These climate cabinets can be used for small scale experiments, seed germination and plant- and animal culturing.

Greenhouse, controlled experiments with herbivores. Photo: IBED

Greenhouse

The greenhouse was opened in 2006 and is used by IBED and SILS. The greenhouse offers excellent conditions for research, and contains many independent units, partly fully climatized, and with the legal permits to do research on genetically modified plants. Also, there are compartments designated for conducting controlled experiments with herbivores.

Organization

Three technicians are dedicated to the climate chambers and insect culture facilities; Taymaz Azarno, Dennis van Veldhuizen and Lin Dong. They are responsible for the climate chambers, moth lab and Thrips&mite labs respectively.

Moreover, they regularly assist in a wide range of projects and courses of Bachelor and Master students.

Contact information

You can contact us if you are a permanent IBED staff member and you or your students are planning to undertake a project for which you need space in a climatized facility.

Please contact us ahead of time!

P. (Peter) Kuperus

Lab Manager

L. (Ludek) Tikovsky

Manager of the Greenhouse facility