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Cell analysis and cell quantification

CASY Cell counter. Photo: IBED

CASY TTT Cell counter

Our lab is equipped with a CASY TTT Cell counter. The CASY measures the conductivity between 2 electrodes separated by a defined pore. Cells are suspended in CASYton, a defined buffer solution. During measurement, cells passing through the pore generate an electrical pulse. The intensity of the signal directly correlates with the volume of the cell. Hence CASY detects the volume of each cell passing through the pore. This allows for the quantification of our algal cultures both in cell number as in biovolume.

MIMS. Photo: IBED

Membrane Inlet Mass Spectrometry (MIMS)

In our laboratory we have a Hiden HPR-40 Membrane Inlet Mass spectrometer installed with a mass range of 200 amu (atomic mass unit) with a dissolved species probe. This allows for accurate measurements of a wide range of gasses either in gaseous or dissolved in liquid environments. Using stable isotopes specific changes in conditions applied can be quantified. The probe is positioned in a specialised cuvette.

Typical applications of the MIMS in our lab are:

  • 13C-labeled bicarbonate or carbon dioxide uptake experiments to quantify rates of carbon fixation in cyanobacteria and algae.
  • 18O2 uptake experiments to quantify oxidation or respiration rates during photosynthesis in cyanbacteria and algae.
  • Technical specifications:
    • Hiden analytical HPR-40 Membrane mass spectrometer
    • HAL201 RC mass spectrometer with Faraday/Multiplier detector
    • Mass range 200 amu
    • Dissolved species probe type 303416

Contact information

Please contact us for more information: IBED-lab-science@uva.nl

Dr. J.M. (Merijn) Schuurmans

Faculty of Science

Institute for Biodiversity and Ecosystem Dynamics