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Microscopy

Fluorescence microscope, Zeiss Axioskop II

This microscope is particularly suitably for fluorescence purposes. Besides chlorophyl autofluorescence, fluorescence with the dyes FISH, SYBR Green and DAPI are the most common used applications.

  • Technical specifications
    • Fluor Zeiss Axioskop II
    • 50W HBO lamp (mercury )
    • 4 filtersets

     

    Excitation

    Beam Splitter

    Emission

    Filter Set 02

    G 365

    FT 395 

    LP 420

    Filter Set 10

    BP 450-490

    FT 510

    BP 515-565

    Filter Set 20

    BP 546/12

    FT 560

    BP 575-640

    Filter Set 26

    BP 575-625

    FT 645

    BP 660-710

    • Camera with Image Focus 4 programme

Inverted microscope DM IRB

The Leica inverted microscope is perfect for examining plankton samples and microscopic slides in bright field with your own two eyes. It is equipped with a camera connected to a computer for taking pictures but also for measuring dimensions of whatever it is you have in front of your lens. It has objectives for both short and long working distance and can produce magnifications from 40 times up to 630 times!

  • Technical specifications
    • Condenser with NA
    • Objectives:
      - C Plan 4x/0.10na
      - N Plan 10×/0.25na
      - N Plan 20x/0.40na
      - HC PlanApo 20×/0.70na
      - N Plan 40x/0.55na
      - HCX Plan Fluotar 40x/0.75na
      - Plan Fluotar 63x/na0.70
      - HCX Plan Fluotar 63×/1.25na (immersion oil)
    • Grey, yellow and blue filters
    • Camera: Lumenera Infinity 1 with Infinity Analyze.
Leica 20EB Dialux microscope

Microscope with Phase contrast and fluorescence possibility

Except normal light, this microscope has a Phase contrast objective and fluorescence options.

Applications:

  • Checking sample slides with normal light
  • Phase contrast (50x). Particularly suitably for checking bacteria.
  • Technical specifications
    • Leica 20EB Dialux microscope
    • 3 filter blocks for fluorescence

      Filterblock

      Excitation (nm)

      Emission (nm)

      Applications

      D

      355-425

      >470

      Chlorophyll (autofluorescence)
      DAPI

      F 100/LP250

      455-495

      512-558

      Cybergreen
      GFP Fluoresceine

      N2.1

      515-560

      >590

      Phycocyanin
      Phycoerythrin
      FITC
      TRITC

    • Camera with Infinity analyse programm        

Contact information

Please contact us for more information: IBED-lab-science@uva.nl

Dr. J.M. (Merijn) Schuurmans

Faculty of Science

Institute for Biodiversity and Ecosystem Dynamics